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KMID : 0918520150150020059
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Journal of the Korean Society of Inherited Metabolic Disease
2015 Volume.15 No. 2 p.59 ~ p.64
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Simultaneous Quantification of Urinary L-, and D-Lactate by Reversed-Phase Liquid Chromatography Tandem Mass Spectrometry
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Moon Chul-Jin
Yang Song-Hyun
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Abstract
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Purpose: Lactate has two optical isomers, L-lactate and D-lactate. In human L-lactate is the most abundant enantiomer of lactate. As plasma and urinary levels of L-lactate is associated with inherited metabolic disorders in general, D-lactate have been linked to the presence of diabetes and inflammatory bowel disease. Previously developed techniques have shown several limitations to further evaluate Dlactate as a biomarker for this condition. In this paper, we describe a highly sensitive, specific and fast liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the analysis of D-, L-lactate in urine.
Methods: D- and L-lactate were quantified using high performance liquid chromatography tandem mass spectrometry (LC-MS/MS) with labelled internal standard. Samples were derivatized with (+)-O,O’- diacety-L-tartaric anhydride (DATAN) and seperated on a Poroshell 120 EC-C18 column.
Results: Quantitative analysis of D-, and L-lactate was achieved successfully. Calibration curves were linear (r>0.999) over 0.5-100 μg/mL. Stabilities for samples were within the 10% varation. Inter- and Intra-day assay variations were below 10%.
Conclusion: The presented method proved to be suitable for the quantitation of D- and L-lactate and opens the possibility to explore the use of D-lactate as a biomarker.
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KEYWORD
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D-lactate, L-Lacate, LC-MS/MS
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